Exploring the Genetic Background of the Differences in Nest-Building Behavior in European Rabbit

  • Once a day, nursing and absentee mothering make the wild rabbit (Oryctolagus cuniculus) an ideal model animal for measuring differences in maternal behavior. Behavioral events and their hormonal regulation leading to parturition are well documented; however, the genetic background behind individual differences in this complex process is unknown.
  • Decreased progesterone hormone level and the reduction of progesterone receptor activity are crucial to initiating the collection of nest material. The progesterone receptor gene is a likely candidate affecting nest-building behavior. In addition to several known point mutations in the progesterone receptor gene of the European wild rabbit, we have found a new mutation in the promoter region of the gene at 2682 T > C.
  • Although this new single nucleotide polymorphism (SNP) was not involved in the formation of the nest-building behavior, an SNP (2464G > A) already described in the promoter region showed an association with individual differences in the initiation of hay carrying. The distribution of this SNP delivered an opposite result compared to domestic rabbits.
  • Genotype (GG) with high uterine capacity was most frequent; the hereditary value of the trait was h2 = 0.10. Thus, progesterone receptor gene polymorphism may manifest in individual differences affecting breeding success in this species.
informedchoiceaboutcancerscreening
informedchoiceaboutcancerscreening

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit 

P-2003
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One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

Easy? One Step RT Kit

M1162-100 each
EUR 561.6

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 288

[One Step] CAT Antibody Kit

RK05630 50 ul
EUR 288

[One Step] GDA Antibody Kit

RK05648 50 ul
EUR 288

[One Step] GPI Antibody Kit

RK05650 50 ul
EUR 288

[One Step] HK1 Antibody Kit

RK05655 50 ul
EUR 288

[One Step] OAT Antibody Kit

RK05674 50 ul
EUR 288

[One Step] PHB Antibody Kit

RK05679 50 ul
EUR 288

[One Step] RAN Antibody Kit

RK05695 50 ul
EUR 288

[One Step] VCP Antibody Kit

RK05721 50 ul
EUR 288

[One Step] MIF antibody Kit

RK05730 50 ul
EUR 288

[One Step] SRM antibody Kit

RK05732 50 ul
EUR 288

[One Step] HK1 Antibody Kit

MBS9145313-002mL 0.02mL
EUR 200

[One Step] HK1 Antibody Kit

MBS9145313-005mL 0.05mL
EUR 255

[One Step] HK1 Antibody Kit

MBS9145313-01mL 0.1mL
EUR 345

[One Step] HK1 Antibody Kit

MBS9145313-02mL 0.2mL
EUR 545

[One Step] HK1 Antibody Kit

MBS9145313-5x02mL 5x0.2mL
EUR 2265

[One Step] MIF antibody Kit

MBS9145322-002mL 0.02mL
EUR 235

[One Step] MIF antibody Kit

MBS9145322-005mL 0.05mL
EUR 295

[One Step] MIF antibody Kit

MBS9145322-01mL 0.1mL
EUR 425

[One Step] MIF antibody Kit

MBS9145322-02mL 0.2mL
EUR 600

[One Step] MIF antibody Kit

MBS9145322-5x02mL 5x0.2mL
EUR 2525

[One Step] RAN Antibody Kit

MBS9145352-002mL 0.02mL
EUR 200

[One Step] RAN Antibody Kit

MBS9145352-005mL 0.05mL
EUR 255

[One Step] RAN Antibody Kit

MBS9145352-01mL 0.1mL
EUR 345

[One Step] RAN Antibody Kit

MBS9145352-02mL 0.2mL
EUR 545

[One Step] RAN Antibody Kit

MBS9145352-5x02mL 5x0.2mL
EUR 2265

[One Step] AK4 Antibody Kit

MBS9145359-002mL 0.02mL
EUR 220

[One Step] AK4 Antibody Kit

MBS9145359-005mL 0.05mL
EUR 295

[One Step] AK4 Antibody Kit

MBS9145359-01mL 0.1mL
EUR 430

[One Step] AK4 Antibody Kit

MBS9145359-02mL 0.2mL
EUR 600

[One Step] AK4 Antibody Kit

MBS9145359-5x02mL 5x0.2mL
EUR 2525

[One Step] PHB Antibody Kit

MBS9145372-002mL 0.02mL
EUR 220

[One Step] PHB Antibody Kit

MBS9145372-005mL 0.05mL
EUR 295

[One Step] PHB Antibody Kit

MBS9145372-01mL 0.1mL
EUR 430

[One Step] PHB Antibody Kit

MBS9145372-02mL 0.2mL
EUR 600

[One Step] PHB Antibody Kit

MBS9145372-5x02mL 5x0.2mL
EUR 2525

[One Step] SRM antibody Kit

MBS9145380-002mL 0.02mL
EUR 220

[One Step] SRM antibody Kit

MBS9145380-005mL 0.05mL
EUR 295

[One Step] SRM antibody Kit

MBS9145380-01mL 0.1mL
EUR 430

[One Step] SRM antibody Kit

MBS9145380-02mL 0.2mL
EUR 600

[One Step] SRM antibody Kit

MBS9145380-5x02mL 5x0.2mL
EUR 2525

[One Step] OAT Antibody Kit

MBS9145397-002mL 0.02mL
EUR 235

[One Step] OAT Antibody Kit

MBS9145397-005mL 0.05mL
EUR 295

[One Step] OAT Antibody Kit

MBS9145397-01mL 0.1mL
EUR 420

[One Step] OAT Antibody Kit

MBS9145397-02mL 0.2mL
EUR 600

[One Step] OAT Antibody Kit

MBS9145397-5x02mL 5x0.2mL
EUR 2525

[One Step] GDA Antibody Kit

MBS9145406-002mL 0.02mL
EUR 200

[One Step] GDA Antibody Kit

MBS9145406-005mL 0.05mL
EUR 255

[One Step] GDA Antibody Kit

MBS9145406-01mL 0.1mL
EUR 345

[One Step] GDA Antibody Kit

MBS9145406-02mL 0.2mL
EUR 545

[One Step] GDA Antibody Kit

MBS9145406-5x02mL 5x0.2mL
EUR 2265

[One Step] GPI Antibody Kit

MBS9145412-002mL 0.02mL
EUR 200

[One Step] GPI Antibody Kit

MBS9145412-005mL 0.05mL
EUR 255

[One Step] GPI Antibody Kit

MBS9145412-01mL 0.1mL
EUR 345

[One Step] GPI Antibody Kit

MBS9145412-02mL 0.2mL
EUR 545

[One Step] GPI Antibody Kit

MBS9145412-5x02mL 5x0.2mL
EUR 2265

[One Step] VCP Antibody Kit

MBS9145423-002mL 0.02mL
EUR 200

[One Step] VCP Antibody Kit

MBS9145423-005mL 0.05mL
EUR 255

[One Step] VCP Antibody Kit

MBS9145423-01mL 0.1mL
EUR 345

[One Step] VCP Antibody Kit

MBS9145423-02mL 0.2mL
EUR 545

[One Step] VCP Antibody Kit

MBS9145423-5x02mL 5x0.2mL
EUR 2265

[One Step] BMP5 Antibody Kit

RK05627 50 ul
EUR 288

[One Step] CBX3 Antibody Kit

RK05631 50 ul
EUR 288

[One Step] CCT8 Antibody Kit

RK05632 50 ul
EUR 288

[One Step] CST3 Antibody Kit

RK05639 50 ul
EUR 288

[One Step] DARS Antibody Kit

RK05641 50 ul
EUR 288

[One Step] FDPS Antibody Kit

RK05646 50 ul
EUR 288

[One Step] GOT2 Antibody Kit

RK05649 50 ul
EUR 288

[One Step] HAX1 Antibody Kit

RK05654 50 ul
EUR 288

[One Step] LSM4 Antibody Kit

RK05663 50 ul
EUR 288

[One Step] ERK2 Antibody Kit

RK05664 50 ul
EUR 288

[One Step] MCM3 Antibody Kit

RK05666 50 ul
EUR 288

[One Step] MOGS Antibody Kit

RK05667 50 ul
EUR 288

[One Step] NFU1 Antibody Kit

RK05673 50 ul
EUR 288

[One Step] PARG Antibody Kit

RK05676 50 ul
EUR 288

[One Step] PDXK Antibody Kit

RK05677 50 ul
EUR 288

[One Step] PIDD Antibody Kit

RK05680 50 ul
EUR 288

[One Step] PPIA Antibody Kit

RK05682 50 ul
EUR 288

[One Step] PYGB Antibody Kit

RK05693 50 ul
EUR 288

[One Step] RALB Antibody Kit

RK05694 50 ul
EUR 288

[One Step] RPA2 Antibody Kit

RK05696 50 ul
EUR 288

[One Step] RPS3 Antibody Kit

RK05703 50 ul
EUR 288

Examination of genetic and pharmacological tools to study the proteasomal deubiquitinating enzyme USP14 in the nervous system

Strategies for enhancing protein degradation have been proposed for treating neurological diseases associated with a decline in proteasome activity. A proteasomal deubiquitinating enzyme that controls substrate entry into proteasomes, ubiquitin-specific protease 14 (USP14), is an attractive candidate for therapies that modulate proteasome activity.
This report tests the validity of genetic and pharmacological tools to study USP14’s role in regulating protein abundance. Although previous studies implicated USP14 in the degradation of MAPT, TARDBP, and PRNP, the levels of these proteins were similar in our neurons cultured from wild type and USP14-deficient mice. Neither loss nor overexpression of USP14 affected the levels of these proteins in mice, implying that modifying the amount of USP14 is not sufficient to alter their steady-state levels.
However, neuronal overexpression of a catalytic mutant of USP14 showed that manipulating USP14’s ubiquitin-hydrolase activity altered the levels of specific proteins in vivo. Although pharmacological inhibitors of USP14’s ubiquitin-hydrolase activity reduced MAPT, TARDBP, and PRNP in culture, the effect was similar in wild type and USP14-deficient neurons, thus impacting their use for specifically evaluating USP14 in a therapeutic manner.
While examining how targeting USP14 may affect other proteins in vivo, this report showed that FASN, RELA, CTNNB1, and SNAP23 are reduced in USP14-deficient mice; however, loss of USP14 differentially altered the levels of these proteins in the liver and brain. As such, it is critical to more thoroughly examine how inhibiting USP14 alters protein abundance to determine if targeting USP14 will be a beneficial strategy for treating neurodegenerative diseases.

Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton.
Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library.
At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

Easy? One Step RT Kit

M1162-100 each
EUR 561.6

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 288

[One Step] CAT Antibody Kit

RK05630 50 ul
EUR 288

[One Step] GDA Antibody Kit

RK05648 50 ul
EUR 288

[One Step] GPI Antibody Kit

RK05650 50 ul
EUR 288

[One Step] HK1 Antibody Kit

RK05655 50 ul
EUR 288

[One Step] OAT Antibody Kit

RK05674 50 ul
EUR 288

[One Step] PHB Antibody Kit

RK05679 50 ul
EUR 288

[One Step] RAN Antibody Kit

RK05695 50 ul
EUR 288

[One Step] VCP Antibody Kit

RK05721 50 ul
EUR 288

[One Step] MIF antibody Kit

RK05730 50 ul
EUR 288

[One Step] SRM antibody Kit

RK05732 50 ul
EUR 288

[One Step] HK1 Antibody Kit

MBS9145313-002mL 0.02mL
EUR 200

[One Step] HK1 Antibody Kit

MBS9145313-005mL 0.05mL
EUR 255

[One Step] HK1 Antibody Kit

MBS9145313-01mL 0.1mL
EUR 345

[One Step] HK1 Antibody Kit

MBS9145313-02mL 0.2mL
EUR 545

[One Step] HK1 Antibody Kit

MBS9145313-5x02mL 5x0.2mL
EUR 2265

[One Step] MIF antibody Kit

MBS9145322-002mL 0.02mL
EUR 235

[One Step] MIF antibody Kit

MBS9145322-005mL 0.05mL
EUR 295

[One Step] MIF antibody Kit

MBS9145322-01mL 0.1mL
EUR 425

[One Step] MIF antibody Kit

MBS9145322-02mL 0.2mL
EUR 600

[One Step] MIF antibody Kit

MBS9145322-5x02mL 5x0.2mL
EUR 2525

[One Step] RAN Antibody Kit

MBS9145352-002mL 0.02mL
EUR 200

[One Step] RAN Antibody Kit

MBS9145352-005mL 0.05mL
EUR 255

[One Step] RAN Antibody Kit

MBS9145352-01mL 0.1mL
EUR 345

[One Step] RAN Antibody Kit

MBS9145352-02mL 0.2mL
EUR 545

[One Step] RAN Antibody Kit

MBS9145352-5x02mL 5x0.2mL
EUR 2265

[One Step] AK4 Antibody Kit

MBS9145359-002mL 0.02mL
EUR 220

[One Step] AK4 Antibody Kit

MBS9145359-005mL 0.05mL
EUR 295

[One Step] AK4 Antibody Kit

MBS9145359-01mL 0.1mL
EUR 430

[One Step] AK4 Antibody Kit

MBS9145359-02mL 0.2mL
EUR 600

[One Step] AK4 Antibody Kit

MBS9145359-5x02mL 5x0.2mL
EUR 2525

[One Step] PHB Antibody Kit

MBS9145372-002mL 0.02mL
EUR 220

[One Step] PHB Antibody Kit

MBS9145372-005mL 0.05mL
EUR 295

[One Step] PHB Antibody Kit

MBS9145372-01mL 0.1mL
EUR 430

[One Step] PHB Antibody Kit

MBS9145372-02mL 0.2mL
EUR 600

[One Step] PHB Antibody Kit

MBS9145372-5x02mL 5x0.2mL
EUR 2525

[One Step] SRM antibody Kit

MBS9145380-002mL 0.02mL
EUR 220

[One Step] SRM antibody Kit

MBS9145380-005mL 0.05mL
EUR 295

[One Step] SRM antibody Kit

MBS9145380-01mL 0.1mL
EUR 430

[One Step] SRM antibody Kit

MBS9145380-02mL 0.2mL
EUR 600

[One Step] SRM antibody Kit

MBS9145380-5x02mL 5x0.2mL
EUR 2525

[One Step] OAT Antibody Kit

MBS9145397-002mL 0.02mL
EUR 235

[One Step] OAT Antibody Kit

MBS9145397-005mL 0.05mL
EUR 295

[One Step] OAT Antibody Kit

MBS9145397-01mL 0.1mL
EUR 420

[One Step] OAT Antibody Kit

MBS9145397-02mL 0.2mL
EUR 600

[One Step] OAT Antibody Kit

MBS9145397-5x02mL 5x0.2mL
EUR 2525

[One Step] GDA Antibody Kit

MBS9145406-002mL 0.02mL
EUR 200

[One Step] GDA Antibody Kit

MBS9145406-005mL 0.05mL
EUR 255

[One Step] GDA Antibody Kit

MBS9145406-01mL 0.1mL
EUR 345

[One Step] GDA Antibody Kit

MBS9145406-02mL 0.2mL
EUR 545

[One Step] GDA Antibody Kit

MBS9145406-5x02mL 5x0.2mL
EUR 2265

[One Step] GPI Antibody Kit

MBS9145412-002mL 0.02mL
EUR 200

[One Step] GPI Antibody Kit

MBS9145412-005mL 0.05mL
EUR 255

[One Step] GPI Antibody Kit

MBS9145412-01mL 0.1mL
EUR 345

[One Step] GPI Antibody Kit

MBS9145412-02mL 0.2mL
EUR 545

[One Step] GPI Antibody Kit

MBS9145412-5x02mL 5x0.2mL
EUR 2265

[One Step] VCP Antibody Kit

MBS9145423-002mL 0.02mL
EUR 200

[One Step] VCP Antibody Kit

MBS9145423-005mL 0.05mL
EUR 255

[One Step] VCP Antibody Kit

MBS9145423-01mL 0.1mL
EUR 345

[One Step] VCP Antibody Kit

MBS9145423-02mL 0.2mL
EUR 545

[One Step] VCP Antibody Kit

MBS9145423-5x02mL 5x0.2mL
EUR 2265

[One Step] BMP5 Antibody Kit

RK05627 50 ul
EUR 288

[One Step] CBX3 Antibody Kit

RK05631 50 ul
EUR 288

[One Step] CCT8 Antibody Kit

RK05632 50 ul
EUR 288

[One Step] CST3 Antibody Kit

RK05639 50 ul
EUR 288

[One Step] DARS Antibody Kit

RK05641 50 ul
EUR 288

[One Step] FDPS Antibody Kit

RK05646 50 ul
EUR 288

[One Step] GOT2 Antibody Kit

RK05649 50 ul
EUR 288

[One Step] HAX1 Antibody Kit

RK05654 50 ul
EUR 288

[One Step] LSM4 Antibody Kit

RK05663 50 ul
EUR 288

[One Step] ERK2 Antibody Kit

RK05664 50 ul
EUR 288

[One Step] MCM3 Antibody Kit

RK05666 50 ul
EUR 288

[One Step] MOGS Antibody Kit

RK05667 50 ul
EUR 288

[One Step] NFU1 Antibody Kit

RK05673 50 ul
EUR 288

[One Step] PARG Antibody Kit

RK05676 50 ul
EUR 288

[One Step] PDXK Antibody Kit

RK05677 50 ul
EUR 288

[One Step] PIDD Antibody Kit

RK05680 50 ul
EUR 288

[One Step] PPIA Antibody Kit

RK05682 50 ul
EUR 288

[One Step] PYGB Antibody Kit

RK05693 50 ul
EUR 288

[One Step] RALB Antibody Kit

RK05694 50 ul
EUR 288

[One Step] RPA2 Antibody Kit

RK05696 50 ul
EUR 288

[One Step] RPS3 Antibody Kit

RK05703 50 ul
EUR 288

[One Step] RRM1 Antibody Kit

RK05705 50 ul
EUR 288

[One Step] SDHB Antibody Kit

RK05707 50 ul
EUR 288

[One Step] SFPQ Antibody Kit

RK05708 50 ul
EUR 288

[One Step] STK4 Antibody Kit

RK05712 50 ul
EUR 288