Exploring the Genetic Background of the Differences in Nest-Building Behavior in European Rabbit

  • Once a day, nursing and absentee mothering make the wild rabbit (Oryctolagus cuniculus) an ideal model animal for measuring differences in maternal behavior. Behavioral events and their hormonal regulation leading to parturition are well documented; however, the genetic background behind individual differences in this complex process is unknown.
  • Decreased progesterone hormone level and the reduction of progesterone receptor activity are crucial to initiating the collection of nest material. The progesterone receptor gene is a likely candidate affecting nest-building behavior. In addition to several known point mutations in the progesterone receptor gene of the European wild rabbit, we have found a new mutation in the promoter region of the gene at 2682 T > C.
  • Although this new single nucleotide polymorphism (SNP) was not involved in the formation of the nest-building behavior, an SNP (2464G > A) already described in the promoter region showed an association with individual differences in the initiation of hay carrying. The distribution of this SNP delivered an opposite result compared to domestic rabbits.
  • Genotype (GG) with high uterine capacity was most frequent; the hereditary value of the trait was h2 = 0.10. Thus, progesterone receptor gene polymorphism may manifest in individual differences affecting breeding success in this species.
informedchoiceaboutcancerscreening
informedchoiceaboutcancerscreening

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit 

P-2003
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

Easy? One Step RT Kit

M1162-100 each
EUR 561.6

[One Step] GPI Antibody Kit

RK05650 50 ul
EUR 288

[One Step] HK1 Antibody Kit

RK05655 50 ul
EUR 288

[One Step] OAT Antibody Kit

RK05674 50 ul
EUR 288

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 288

[One Step] CAT Antibody Kit

RK05630 50 ul
EUR 288

[One Step] GDA Antibody Kit

RK05648 50 ul
EUR 288

[One Step] PHB Antibody Kit

RK05679 50 ul
EUR 288

[One Step] RAN Antibody Kit

RK05695 50 ul
EUR 288

[One Step] VCP Antibody Kit

RK05721 50 ul
EUR 288

[One Step] MIF antibody Kit

RK05730 50 ul
EUR 288

[One Step] SRM antibody Kit

RK05732 50 ul
EUR 288

[One Step] GOT2 Antibody Kit

RK05649 50 ul
EUR 288

[One Step] HAX1 Antibody Kit

RK05654 50 ul
EUR 288

[One Step] LSM4 Antibody Kit

RK05663 50 ul
EUR 288

[One Step] NFU1 Antibody Kit

RK05673 50 ul
EUR 288

[One Step] RPS3 Antibody Kit

RK05703 50 ul
EUR 288

[One Step] RRM1 Antibody Kit

RK05705 50 ul
EUR 288

[One Step] SDHB Antibody Kit

RK05707 50 ul
EUR 288

[One Step] SFPQ Antibody Kit

RK05708 50 ul
EUR 288

[One Step] STK4 Antibody Kit

RK05712 50 ul
EUR 288

[One Step] WARS Antibody Kit

RK05722 50 ul
EUR 288

[One Step] BMP5 Antibody Kit

RK05627 50 ul
EUR 288

[One Step] CBX3 Antibody Kit

RK05631 50 ul
EUR 288

[One Step] CCT8 Antibody Kit

RK05632 50 ul
EUR 288

[One Step] CST3 Antibody Kit

RK05639 50 ul
EUR 288

[One Step] DARS Antibody Kit

RK05641 50 ul
EUR 288

[One Step] FDPS Antibody Kit

RK05646 50 ul
EUR 288

[One Step] ERK2 Antibody Kit

RK05664 50 ul
EUR 288

[One Step] MCM3 Antibody Kit

RK05666 50 ul
EUR 288

[One Step] MOGS Antibody Kit

RK05667 50 ul
EUR 288

[One Step] PARG Antibody Kit

RK05676 50 ul
EUR 288

[One Step] PDXK Antibody Kit

RK05677 50 ul
EUR 288

[One Step] PIDD Antibody Kit

RK05680 50 ul
EUR 288

[One Step] PPIA Antibody Kit

RK05682 50 ul
EUR 288

[One Step] PYGB Antibody Kit

RK05693 50 ul
EUR 288

[One Step] RALB Antibody Kit

RK05694 50 ul
EUR 288

[One Step] RPA2 Antibody Kit

RK05696 50 ul
EUR 288

[One Step] TOP1 Antibody Kit

RK05716 50 ul
EUR 288

[One Step] TUFM Antibody Kit

RK05717 50 ul
EUR 288

[One Step] RPS5 antibody Kit

RK05729 50 ul
EUR 288

[One Step] LDHB antibody Kit

RK05731 50 ul
EUR 288

[One Step] GARS antibody Kit

RK05739 50 ul
EUR 288

[One Step] ALDOA Antibody Kit

RK05621 50 ul
EUR 288

[One Step] ALDOB Antibody Kit

RK05622 50 ul
EUR 288

[One Step] ATP5H Antibody Kit

RK05625 50 ul
EUR 288

[One Step] DISC1 Antibody Kit

RK05642 50 ul
EUR 288

[One Step] EIF5A Antibody Kit

RK05645 50 ul
EUR 288

[One Step] GSTP1 Antibody Kit

RK05651 50 ul
EUR 288

[One Step] HACE1 Antibody Kit

RK05652 50 ul
EUR 288

[One Step] HADHA Antibody Kit

RK05653 50 ul
EUR 288

[One Step] HPRT1 Antibody Kit

RK05657 50 ul
EUR 288

[One Step] HSP70 Antibody Kit

RK05659 50 ul
EUR 288

[One Step] Hsc70 Antibody Kit

RK05660 50 ul
EUR 288

[One Step] Hsp60 Antibody Kit

RK05661 50 ul
EUR 288

[One Step] KPNB1 Antibody Kit

RK05662 50 ul
EUR 288

[One Step] RPL36 Antibody Kit

RK05700 50 ul
EUR 288

[One Step] RPS10 Antibody Kit

RK05701 50 ul
EUR 288

[One Step] RPS14 Antibody Kit

RK05702 50 ul
EUR 288

[One Step] RPS4X Antibody Kit

RK05704 50 ul
EUR 288

[One Step] SRSF3 Antibody Kit

RK05710 50 ul
EUR 288

[One Step] TCF12 Antibody Kit

RK05714 50 ul
EUR 288

[One Step] YWHAB Antibody Kit

RK05723 50 ul
EUR 288

[One Step] YWHAZ Antibody Kit

RK05724 50 ul
EUR 288

[One Step] PRDX2 antibody Kit

RK05725 50 ul
EUR 288

[One Step] PRDX3 antibody Kit

RK05726 50 ul
EUR 288

[One Step] RAB7A antibody Kit

RK05740 50 ul
EUR 288

[One Step] ACTR2 Antibody Kit

RK05617 50 ul
EUR 288

[One Step] ACTR3 Antibody Kit

RK05618 50 ul
EUR 288

[One Step] C1QBP Antibody Kit

RK05628 50 ul
EUR 288

[One Step] CALB2 Antibody Kit

RK05629 50 ul
EUR 288

[One Step] CLDN7 Antibody Kit

RK05634 50 ul
EUR 288

[One Step] COPS5 Antibody Kit

RK05635 50 ul
EUR 288

[One Step] COX5A Antibody Kit

RK05636 50 ul
EUR 288

[One Step] COX5B Antibody Kit

RK05637 50 ul
EUR 288

[One Step] FLOT1 Antibody Kit

RK05647 50 ul
EUR 288

[One Step] MCCC2 Antibody Kit

RK05665 50 ul
EUR 288

[One Step] PEBP1 Antibody Kit

RK05678 50 ul
EUR 288

[One Step] PQBP1 Antibody Kit

RK05684 50 ul
EUR 288

[One Step] PSMA2 Antibody Kit

RK05685 50 ul
EUR 288

[One Step] PSMA3 Antibody Kit

RK05686 50 ul
EUR 288

[One Step] PSMA4 Antibody Kit

RK05687 50 ul
EUR 288

[One Step] PSMA5 Antibody Kit

RK05688 50 ul
EUR 288

Examination of genetic and pharmacological tools to study the proteasomal deubiquitinating enzyme USP14 in the nervous system

Strategies for enhancing protein degradation have been proposed for treating neurological diseases associated with a decline in proteasome activity. A proteasomal deubiquitinating enzyme that controls substrate entry into proteasomes, ubiquitin-specific protease 14 (USP14), is an attractive candidate for therapies that modulate proteasome activity.
This report tests the validity of genetic and pharmacological tools to study USP14’s role in regulating protein abundance. Although previous studies implicated USP14 in the degradation of MAPT, TARDBP, and PRNP, the levels of these proteins were similar in our neurons cultured from wild type and USP14-deficient mice. Neither loss nor overexpression of USP14 affected the levels of these proteins in mice, implying that modifying the amount of USP14 is not sufficient to alter their steady-state levels.
However, neuronal overexpression of a catalytic mutant of USP14 showed that manipulating USP14’s ubiquitin-hydrolase activity altered the levels of specific proteins in vivo. Although pharmacological inhibitors of USP14’s ubiquitin-hydrolase activity reduced MAPT, TARDBP, and PRNP in culture, the effect was similar in wild type and USP14-deficient neurons, thus impacting their use for specifically evaluating USP14 in a therapeutic manner.
While examining how targeting USP14 may affect other proteins in vivo, this report showed that FASN, RELA, CTNNB1, and SNAP23 are reduced in USP14-deficient mice; however, loss of USP14 differentially altered the levels of these proteins in the liver and brain. As such, it is critical to more thoroughly examine how inhibiting USP14 alters protein abundance to determine if targeting USP14 will be a beneficial strategy for treating neurodegenerative diseases.

Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton.
Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library.
At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

Easy? One Step RT Kit

M1162-100 each
EUR 561.6

[One Step] GPI Antibody Kit

RK05650 50 ul
EUR 288

[One Step] HK1 Antibody Kit

RK05655 50 ul
EUR 288

[One Step] OAT Antibody Kit

RK05674 50 ul
EUR 288

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 288

[One Step] CAT Antibody Kit

RK05630 50 ul
EUR 288

[One Step] GDA Antibody Kit

RK05648 50 ul
EUR 288

[One Step] PHB Antibody Kit

RK05679 50 ul
EUR 288

[One Step] RAN Antibody Kit

RK05695 50 ul
EUR 288

[One Step] VCP Antibody Kit

RK05721 50 ul
EUR 288

[One Step] MIF antibody Kit

RK05730 50 ul
EUR 288

[One Step] SRM antibody Kit

RK05732 50 ul
EUR 288

[One Step] GOT2 Antibody Kit

RK05649 50 ul
EUR 288

[One Step] HAX1 Antibody Kit

RK05654 50 ul
EUR 288

[One Step] LSM4 Antibody Kit

RK05663 50 ul
EUR 288

[One Step] NFU1 Antibody Kit

RK05673 50 ul
EUR 288

[One Step] RPS3 Antibody Kit

RK05703 50 ul
EUR 288

[One Step] RRM1 Antibody Kit

RK05705 50 ul
EUR 288

[One Step] SDHB Antibody Kit

RK05707 50 ul
EUR 288

[One Step] SFPQ Antibody Kit

RK05708 50 ul
EUR 288

[One Step] STK4 Antibody Kit

RK05712 50 ul
EUR 288

[One Step] WARS Antibody Kit

RK05722 50 ul
EUR 288

[One Step] BMP5 Antibody Kit

RK05627 50 ul
EUR 288

[One Step] CBX3 Antibody Kit

RK05631 50 ul
EUR 288

[One Step] CCT8 Antibody Kit

RK05632 50 ul
EUR 288

[One Step] CST3 Antibody Kit

RK05639 50 ul
EUR 288

[One Step] DARS Antibody Kit

RK05641 50 ul
EUR 288

[One Step] FDPS Antibody Kit

RK05646 50 ul
EUR 288

[One Step] ERK2 Antibody Kit

RK05664 50 ul
EUR 288

[One Step] MCM3 Antibody Kit

RK05666 50 ul
EUR 288

[One Step] MOGS Antibody Kit

RK05667 50 ul
EUR 288

[One Step] PARG Antibody Kit

RK05676 50 ul
EUR 288

[One Step] PDXK Antibody Kit

RK05677 50 ul
EUR 288

[One Step] PIDD Antibody Kit

RK05680 50 ul
EUR 288

[One Step] PPIA Antibody Kit

RK05682 50 ul
EUR 288

[One Step] PYGB Antibody Kit

RK05693 50 ul
EUR 288

[One Step] RALB Antibody Kit

RK05694 50 ul
EUR 288

[One Step] RPA2 Antibody Kit

RK05696 50 ul
EUR 288

[One Step] TOP1 Antibody Kit

RK05716 50 ul
EUR 288

[One Step] TUFM Antibody Kit

RK05717 50 ul
EUR 288

[One Step] RPS5 antibody Kit

RK05729 50 ul
EUR 288

[One Step] LDHB antibody Kit

RK05731 50 ul
EUR 288

[One Step] GARS antibody Kit

RK05739 50 ul
EUR 288

[One Step] ALDOA Antibody Kit

RK05621 50 ul
EUR 288

[One Step] ALDOB Antibody Kit

RK05622 50 ul
EUR 288

[One Step] ATP5H Antibody Kit

RK05625 50 ul
EUR 288

[One Step] DISC1 Antibody Kit

RK05642 50 ul
EUR 288

[One Step] EIF5A Antibody Kit

RK05645 50 ul
EUR 288

[One Step] GSTP1 Antibody Kit

RK05651 50 ul
EUR 288

[One Step] HACE1 Antibody Kit

RK05652 50 ul
EUR 288

[One Step] HADHA Antibody Kit

RK05653 50 ul
EUR 288

[One Step] HPRT1 Antibody Kit

RK05657 50 ul
EUR 288

[One Step] HSP70 Antibody Kit

RK05659 50 ul
EUR 288

[One Step] Hsc70 Antibody Kit

RK05660 50 ul
EUR 288

[One Step] Hsp60 Antibody Kit

RK05661 50 ul
EUR 288

[One Step] KPNB1 Antibody Kit

RK05662 50 ul
EUR 288

[One Step] RPL36 Antibody Kit

RK05700 50 ul
EUR 288

[One Step] RPS10 Antibody Kit

RK05701 50 ul
EUR 288

[One Step] RPS14 Antibody Kit

RK05702 50 ul
EUR 288

[One Step] RPS4X Antibody Kit

RK05704 50 ul
EUR 288

[One Step] SRSF3 Antibody Kit

RK05710 50 ul
EUR 288

[One Step] TCF12 Antibody Kit

RK05714 50 ul
EUR 288

[One Step] YWHAB Antibody Kit

RK05723 50 ul
EUR 288

[One Step] YWHAZ Antibody Kit

RK05724 50 ul
EUR 288

[One Step] PRDX2 antibody Kit

RK05725 50 ul
EUR 288

[One Step] PRDX3 antibody Kit

RK05726 50 ul
EUR 288

[One Step] RAB7A antibody Kit

RK05740 50 ul
EUR 288

[One Step] ACTR2 Antibody Kit

RK05617 50 ul
EUR 288

[One Step] ACTR3 Antibody Kit

RK05618 50 ul
EUR 288

[One Step] C1QBP Antibody Kit

RK05628 50 ul
EUR 288

[One Step] CALB2 Antibody Kit

RK05629 50 ul
EUR 288

[One Step] CLDN7 Antibody Kit

RK05634 50 ul
EUR 288

[One Step] COPS5 Antibody Kit

RK05635 50 ul
EUR 288

[One Step] COX5A Antibody Kit

RK05636 50 ul
EUR 288

[One Step] COX5B Antibody Kit

RK05637 50 ul
EUR 288

[One Step] FLOT1 Antibody Kit

RK05647 50 ul
EUR 288

[One Step] MCCC2 Antibody Kit

RK05665 50 ul
EUR 288

[One Step] PEBP1 Antibody Kit

RK05678 50 ul
EUR 288

[One Step] PQBP1 Antibody Kit

RK05684 50 ul
EUR 288

[One Step] PSMA2 Antibody Kit

RK05685 50 ul
EUR 288

[One Step] PSMA3 Antibody Kit

RK05686 50 ul
EUR 288

[One Step] PSMA4 Antibody Kit

RK05687 50 ul
EUR 288

[One Step] PSMA5 Antibody Kit

RK05688 50 ul
EUR 288

[One Step] PSMA6 Antibody Kit

RK05689 50 ul
EUR 288

[One Step] PSMB4 Antibody Kit

RK05690 50 ul
EUR 288

[One Step] PSMC2 Antibody Kit

RK05691 50 ul
EUR 288

[One Step] RPL13 Antibody Kit

RK05697 50 ul
EUR 288