Exploring the Genetic Background of the Differences in Nest-Building Behavior in European Rabbit

  • Once a day, nursing and absentee mothering make the wild rabbit (Oryctolagus cuniculus) an ideal model animal for measuring differences in maternal behavior. Behavioral events and their hormonal regulation leading to parturition are well documented; however, the genetic background behind individual differences in this complex process is unknown.
  • Decreased progesterone hormone level and the reduction of progesterone receptor activity are crucial to initiating the collection of nest material. The progesterone receptor gene is a likely candidate affecting nest-building behavior. In addition to several known point mutations in the progesterone receptor gene of the European wild rabbit, we have found a new mutation in the promoter region of the gene at 2682 T > C.
  • Although this new single nucleotide polymorphism (SNP) was not involved in the formation of the nest-building behavior, an SNP (2464G > A) already described in the promoter region showed an association with individual differences in the initiation of hay carrying. The distribution of this SNP delivered an opposite result compared to domestic rabbits.
  • Genotype (GG) with high uterine capacity was most frequent; the hereditary value of the trait was h2 = 0.10. Thus, progesterone receptor gene polymorphism may manifest in individual differences affecting breeding success in this species.
informedchoiceaboutcancerscreening
informedchoiceaboutcancerscreening

ChromaFlash High-Sensitivity ChIP Kit  

P-2027
  • EUR 771.66
  • EUR 338.80
  • EUR 512.60
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

ChromaFlash Chromatin Extraction Kit 

P-2001-100 100 Extractions
EUR 182.6

ChromaFlash Chromatin Extraction Kit 

P-2001
  • EUR 312.30
  • EUR 182.60
  • 100 Extractions
  • 100 Extractions

ChromaFlash Plant Chromatin Extraction Kit 

P-2022-050 50 Extractions
EUR 193.6

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023-100 100 Preparations
EUR 189.2

ChromaFlash Plant Chromatin Extraction Kit 

P-2022
  • EUR 331.44
  • EUR 193.60
  • 50 Extractions
  • 50 Extractions

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

CHIP protein

30R-1245 100 ug
EUR 268.8
Description: Purified recombinant Human CHIP protein

CHIP protein

30R-2753 25 ug
EUR 510
Description: Purified recombinant Human CHIP protein

CHIP Peptide

45-398P 0.1 mg
EUR 405.6
Description: STUB1 / CHIP Peptide

EpiQuik Plant ChIP Kit 

P-2014-24 24 Reactions
EUR 387.2

EpiQuik Plant ChIP Kit 

P-2014-48 48 Reactions
EUR 639.1

EpiQuik Plant ChIP Kit 

P-2014
  • EUR 945.66
  • EUR 387.20
  • EUR 639.10
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

Pospiviroid One-Step PCR kit

Oneq-A546-100R 100T
EUR 1932

Pospiviroid One-Step PCR kit

Oneq-A546-150R 150T
EUR 2646

Pospiviroid One-Step PCR kit

Oneq-A546-50R 50T
EUR 1410

Lactobacillus One-Step PCR kit

Oneq-A566-100D 100T
EUR 1039.2

Lactobacillus One-Step PCR kit

Oneq-A566-150D 150T
EUR 1177.2

Lactobacillus One-Step PCR kit

Oneq-A566-50D 50T
EUR 861.6

Lactococcus One-Step PCR kit

Oneq-A567-100D 100T
EUR 1039.2

Lactococcus One-Step PCR kit

Oneq-A567-150D 150T
EUR 1177.2

Lactococcus One-Step PCR kit

Oneq-A567-50D 50T
EUR 861.6

Leuconostoc One-Step PCR kit

Oneq-A569-100D 100T
EUR 1039.2

Leuconostoc One-Step PCR kit

Oneq-A569-150D 150T
EUR 1177.2

Leuconostoc One-Step PCR kit

Oneq-A569-50D 50T
EUR 861.6

Pediococcus One-Step PCR kit

Oneq-A571-100D 100T
EUR 1039.2

Pediococcus One-Step PCR kit

Oneq-A571-150D 150T
EUR 1177.2

Pediococcus One-Step PCR kit

Oneq-A571-50D 50T
EUR 861.6

Halobacillus One-Step PCR kit

Oneq-A635-100D 100T
EUR 1039.2

Halobacillus One-Step PCR kit

Oneq-A635-150D 150T
EUR 1177.2

Halobacillus One-Step PCR kit

Oneq-A635-50D 50T
EUR 861.6

Enterovirus One-Step PCR kit

Oneq-H433-100R 100T
EUR 1932

Enterovirus One-Step PCR kit

Oneq-H433-150R 150T
EUR 2646

Enterovirus One-Step PCR kit

Oneq-H433-50R 50T
EUR 1410

Mengovirus One-Step PCR kit

Oneq-H453-100R 100T
EUR 1932

Mengovirus One-Step PCR kit

Oneq-H453-150R 150T
EUR 2646

Mengovirus One-Step PCR kit

Oneq-H453-50R 50T
EUR 1410

Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
EUR 1932

Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
EUR 2646

Astrovirus One-Step PCR kit

Oneq-H491-50R 50T
EUR 1410

Calicivirus One-Step PCR kit

Oneq-H493-100R 100T
EUR 1932

Calicivirus One-Step PCR kit

Oneq-H493-150R 150T
EUR 2646

Calicivirus One-Step PCR kit

Oneq-H493-50R 50T
EUR 1410

Adenovirus One-Step PCR kit

Oneq-H501-100D 100T
EUR 1039.2

Adenovirus One-Step PCR kit

Oneq-H501-150D 150T
EUR 1177.2

Adenovirus One-Step PCR kit

Oneq-H501-50D 50T
EUR 861.6

Polyomavirus One-Step PCR kit

Oneq-H515-100D 100T
EUR 1039.2

Polyomavirus One-Step PCR kit

Oneq-H515-150D 150T
EUR 1177.2

Polyomavirus One-Step PCR kit

Oneq-H515-50D 50T
EUR 861.6

Ruminococcus One-Step PCR kit

Oneq-H619-100D 100T
EUR 1039.2

Ruminococcus One-Step PCR kit

Oneq-H619-150D 150T
EUR 1177.2

Ruminococcus One-Step PCR kit

Oneq-H619-50D 50T
EUR 861.6

Bifidobacterium One-Step PCR kit

Oneq-H621-100D 100T
EUR 1039.2

Bifidobacterium One-Step PCR kit

Oneq-H621-150D 150T
EUR 1177.2

Bifidobacterium One-Step PCR kit

Oneq-H621-50D 50T
EUR 861.6

Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
EUR 1932

Sapovirus One-Step PCR kit

Oneq-H630-150R 150T
EUR 2646

Sapovirus One-Step PCR kit

Oneq-H630-50R 50T
EUR 1410

Parechovirus One-Step PCR kit

Oneq-H642-100R 100T
EUR 1932

Parechovirus One-Step PCR kit

Oneq-H642-150R 150T
EUR 2646

Parechovirus One-Step PCR kit

Oneq-H642-50R 50T
EUR 1410

Metapneumovirus One-Step PCR kit

Oneq-H643-100R 100T
EUR 1932

Metapneumovirus One-Step PCR kit

Oneq-H643-150R 150T
EUR 2646

Metapneumovirus One-Step PCR kit

Oneq-H643-50R 50T
EUR 1410

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 1039.2

Entamoeba One-Step PCR kit

Oneq-H683-150D 150T
EUR 1177.2

Entamoeba One-Step PCR kit

Oneq-H683-50D 50T
EUR 861.6

Acanthamoeba One-Step PCR kit

Oneq-H684-100D 100T
EUR 1039.2

Acanthamoeba One-Step PCR kit

Oneq-H684-150D 150T
EUR 1177.2

Acanthamoeba One-Step PCR kit

Oneq-H684-50D 50T
EUR 861.6

Echovirus One-Step PCR kit

Oneq-H722-100D 100T
EUR 1039.2

Echovirus One-Step PCR kit

Oneq-H722-150D 150T
EUR 1177.2

Echovirus One-Step PCR kit

Oneq-H722-50D 50T
EUR 861.6

ranavirus One-Step PCR kit

Oneq-V337-100D 100T
EUR 1039.2

ranavirus One-Step PCR kit

Oneq-V337-150D 150T
EUR 1177.2

ranavirus One-Step PCR kit

Oneq-V337-50D 50T
EUR 861.6

Ruminococcus One-Step PCR kit

Oneq-V619-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit

Oneq-V621-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

One-Step RT-PCR Kit

G174 100 x 50 ul reactions
EUR 279.6

One Step Mouse Genotyping Kit

PD101-01 200 rxn
EUR 259.2

Examination of genetic and pharmacological tools to study the proteasomal deubiquitinating enzyme USP14 in the nervous system

Strategies for enhancing protein degradation have been proposed for treating neurological diseases associated with a decline in proteasome activity. A proteasomal deubiquitinating enzyme that controls substrate entry into proteasomes, ubiquitin-specific protease 14 (USP14), is an attractive candidate for therapies that modulate proteasome activity.
This report tests the validity of genetic and pharmacological tools to study USP14’s role in regulating protein abundance. Although previous studies implicated USP14 in the degradation of MAPT, TARDBP, and PRNP, the levels of these proteins were similar in our neurons cultured from wild type and USP14-deficient mice. Neither loss nor overexpression of USP14 affected the levels of these proteins in mice, implying that modifying the amount of USP14 is not sufficient to alter their steady-state levels.
However, neuronal overexpression of a catalytic mutant of USP14 showed that manipulating USP14’s ubiquitin-hydrolase activity altered the levels of specific proteins in vivo. Although pharmacological inhibitors of USP14’s ubiquitin-hydrolase activity reduced MAPT, TARDBP, and PRNP in culture, the effect was similar in wild type and USP14-deficient neurons, thus impacting their use for specifically evaluating USP14 in a therapeutic manner.
While examining how targeting USP14 may affect other proteins in vivo, this report showed that FASN, RELA, CTNNB1, and SNAP23 are reduced in USP14-deficient mice; however, loss of USP14 differentially altered the levels of these proteins in the liver and brain. As such, it is critical to more thoroughly examine how inhibiting USP14 alters protein abundance to determine if targeting USP14 will be a beneficial strategy for treating neurodegenerative diseases.

Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton.
Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library.
At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

ChromaFlash Chromatin Extraction Kit 

P-2001
  • EUR 312.30
  • EUR 182.60
  • 100 Extractions
  • 100 Extractions

ChromaFlash Plant Chromatin Extraction Kit 

P-2022-050 50 Extractions
EUR 193.6

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023-100 100 Preparations
EUR 189.2

ChromaFlash Plant Chromatin Extraction Kit 

P-2022
  • EUR 331.44
  • EUR 193.60
  • 50 Extractions
  • 50 Extractions

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

CHIP protein

30R-1245 100 ug
EUR 268.8
Description: Purified recombinant Human CHIP protein

CHIP protein

30R-2753 25 ug
EUR 510
Description: Purified recombinant Human CHIP protein

CHIP Peptide

45-398P 0.1 mg
EUR 405.6
Description: STUB1 / CHIP Peptide

EpiQuik Plant ChIP Kit 

P-2014-24 24 Reactions
EUR 387.2

EpiQuik Plant ChIP Kit 

P-2014-48 48 Reactions
EUR 639.1

EpiQuik Plant ChIP Kit 

P-2014
  • EUR 945.66
  • EUR 387.20
  • EUR 639.10
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

Pospiviroid One-Step PCR kit

Oneq-A546-100R 100T
EUR 1932

Pospiviroid One-Step PCR kit

Oneq-A546-150R 150T
EUR 2646

Pospiviroid One-Step PCR kit

Oneq-A546-50R 50T
EUR 1410

Lactobacillus One-Step PCR kit

Oneq-A566-100D 100T
EUR 1039.2

Lactobacillus One-Step PCR kit

Oneq-A566-150D 150T
EUR 1177.2

Lactobacillus One-Step PCR kit

Oneq-A566-50D 50T
EUR 861.6

Lactococcus One-Step PCR kit

Oneq-A567-100D 100T
EUR 1039.2

Lactococcus One-Step PCR kit

Oneq-A567-150D 150T
EUR 1177.2

Lactococcus One-Step PCR kit

Oneq-A567-50D 50T
EUR 861.6

Leuconostoc One-Step PCR kit

Oneq-A569-100D 100T
EUR 1039.2

Leuconostoc One-Step PCR kit

Oneq-A569-150D 150T
EUR 1177.2

Leuconostoc One-Step PCR kit

Oneq-A569-50D 50T
EUR 861.6

Pediococcus One-Step PCR kit

Oneq-A571-100D 100T
EUR 1039.2

Pediococcus One-Step PCR kit

Oneq-A571-150D 150T
EUR 1177.2

Pediococcus One-Step PCR kit

Oneq-A571-50D 50T
EUR 861.6

Halobacillus One-Step PCR kit

Oneq-A635-100D 100T
EUR 1039.2

Halobacillus One-Step PCR kit

Oneq-A635-150D 150T
EUR 1177.2

Halobacillus One-Step PCR kit

Oneq-A635-50D 50T
EUR 861.6

Enterovirus One-Step PCR kit

Oneq-H433-100R 100T
EUR 1932

Enterovirus One-Step PCR kit

Oneq-H433-150R 150T
EUR 2646

Enterovirus One-Step PCR kit

Oneq-H433-50R 50T
EUR 1410

Mengovirus One-Step PCR kit

Oneq-H453-100R 100T
EUR 1932

Mengovirus One-Step PCR kit

Oneq-H453-150R 150T
EUR 2646

Mengovirus One-Step PCR kit

Oneq-H453-50R 50T
EUR 1410

Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
EUR 1932

Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
EUR 2646

Astrovirus One-Step PCR kit

Oneq-H491-50R 50T
EUR 1410

Calicivirus One-Step PCR kit

Oneq-H493-100R 100T
EUR 1932

Calicivirus One-Step PCR kit

Oneq-H493-150R 150T
EUR 2646

Calicivirus One-Step PCR kit

Oneq-H493-50R 50T
EUR 1410

Adenovirus One-Step PCR kit

Oneq-H501-100D 100T
EUR 1039.2

Adenovirus One-Step PCR kit

Oneq-H501-150D 150T
EUR 1177.2

Adenovirus One-Step PCR kit

Oneq-H501-50D 50T
EUR 861.6

Polyomavirus One-Step PCR kit

Oneq-H515-100D 100T
EUR 1039.2

Polyomavirus One-Step PCR kit

Oneq-H515-150D 150T
EUR 1177.2

Polyomavirus One-Step PCR kit

Oneq-H515-50D 50T
EUR 861.6

Ruminococcus One-Step PCR kit

Oneq-H619-100D 100T
EUR 1039.2

Ruminococcus One-Step PCR kit

Oneq-H619-150D 150T
EUR 1177.2

Ruminococcus One-Step PCR kit

Oneq-H619-50D 50T
EUR 861.6

Bifidobacterium One-Step PCR kit

Oneq-H621-100D 100T
EUR 1039.2

Bifidobacterium One-Step PCR kit

Oneq-H621-150D 150T
EUR 1177.2

Bifidobacterium One-Step PCR kit

Oneq-H621-50D 50T
EUR 861.6

Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
EUR 1932

Sapovirus One-Step PCR kit

Oneq-H630-150R 150T
EUR 2646

Sapovirus One-Step PCR kit

Oneq-H630-50R 50T
EUR 1410

Parechovirus One-Step PCR kit

Oneq-H642-100R 100T
EUR 1932

Parechovirus One-Step PCR kit

Oneq-H642-150R 150T
EUR 2646

Parechovirus One-Step PCR kit

Oneq-H642-50R 50T
EUR 1410

Metapneumovirus One-Step PCR kit

Oneq-H643-100R 100T
EUR 1932

Metapneumovirus One-Step PCR kit

Oneq-H643-150R 150T
EUR 2646

Metapneumovirus One-Step PCR kit

Oneq-H643-50R 50T
EUR 1410

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 1039.2

Entamoeba One-Step PCR kit

Oneq-H683-150D 150T
EUR 1177.2

Entamoeba One-Step PCR kit

Oneq-H683-50D 50T
EUR 861.6

Acanthamoeba One-Step PCR kit

Oneq-H684-100D 100T
EUR 1039.2

Acanthamoeba One-Step PCR kit

Oneq-H684-150D 150T
EUR 1177.2

Acanthamoeba One-Step PCR kit

Oneq-H684-50D 50T
EUR 861.6

Echovirus One-Step PCR kit

Oneq-H722-100D 100T
EUR 1039.2

Echovirus One-Step PCR kit

Oneq-H722-150D 150T
EUR 1177.2

Echovirus One-Step PCR kit

Oneq-H722-50D 50T
EUR 861.6

ranavirus One-Step PCR kit

Oneq-V337-100D 100T
EUR 1039.2

ranavirus One-Step PCR kit

Oneq-V337-150D 150T
EUR 1177.2

ranavirus One-Step PCR kit

Oneq-V337-50D 50T
EUR 861.6

Ruminococcus One-Step PCR kit

Oneq-V619-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit

Oneq-V621-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

One-Step RT-PCR Kit

G174 100 x 50 ul reactions
EUR 279.6

One Step Mouse Genotyping Kit

PD101-01 200 rxn
EUR 259.2

[One Step] ACTR2 Antibody Kit

RK05617 50 ul
EUR 288

[One Step] ACTR3 Antibody Kit

RK05618 50 ul
EUR 288

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 288

[One Step] AKR1A1 Antibody Kit

RK05620 50 ul
EUR 288