Exploring the Genetic Background of the Differences in Nest-Building Behavior in European Rabbit

  • Once a day, nursing and absentee mothering make the wild rabbit (Oryctolagus cuniculus) an ideal model animal for measuring differences in maternal behavior. Behavioral events and their hormonal regulation leading to parturition are well documented; however, the genetic background behind individual differences in this complex process is unknown.
  • Decreased progesterone hormone level and the reduction of progesterone receptor activity are crucial to initiating the collection of nest material. The progesterone receptor gene is a likely candidate affecting nest-building behavior. In addition to several known point mutations in the progesterone receptor gene of the European wild rabbit, we have found a new mutation in the promoter region of the gene at 2682 T > C.
  • Although this new single nucleotide polymorphism (SNP) was not involved in the formation of the nest-building behavior, an SNP (2464G > A) already described in the promoter region showed an association with individual differences in the initiation of hay carrying. The distribution of this SNP delivered an opposite result compared to domestic rabbits.
  • Genotype (GG) with high uterine capacity was most frequent; the hereditary value of the trait was h2 = 0.10. Thus, progesterone receptor gene polymorphism may manifest in individual differences affecting breeding success in this species.
informedchoiceaboutcancerscreening
informedchoiceaboutcancerscreening

CHIP Peptide

45-398P 0.1 mg
EUR 338
Description: STUB1 / CHIP Peptide

EpiQuik Plant ChIP Kit

P-2014 48 Reactions
EUR 788.05
Description: Ask the seller for details

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-10ug 10ug
EUR 156
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-1mg 1mg
EUR 2283
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-500ug 500ug
EUR 1613
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-50ug 50ug
EUR 369
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

CHIP Polyclonal Antibody

ES11301-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against CHIP from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

CHIP Polyclonal Antibody

ES11301-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against CHIP from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Human CHIP Antibody

32585-05111 150 ug
EUR 261

Anti-CHIP antibody

STJ192459 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to CHIP

CHIP Polyclonal Antibody

ABP58142-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of CHIP from Human, Mouse. This CHIP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHIP protein at amino acid sequence of 160-240

CHIP Polyclonal Antibody

ABP58142-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of CHIP from Human, Mouse. This CHIP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHIP protein at amino acid sequence of 160-240

CHIP Polyclonal Antibody

ABP58142-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of CHIP from Human, Mouse. This CHIP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHIP protein at amino acid sequence of 160-240

CHIP, human recombinant

4898-25
EUR 349

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

P-2002 96 Reactions
EUR 904.05
Description: Ask the seller for details

[One Step] ACTR2 Antibody Kit

RK05617 50 ul
EUR 240

[One Step] ACTR3 Antibody Kit

RK05618 50 ul
EUR 240

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 240

[One Step] AKR1A1 Antibody Kit

RK05620 50 ul
EUR 240

[One Step] ALDOA Antibody Kit

RK05621 50 ul
EUR 240

[One Step] ALDOB Antibody Kit

RK05622 50 ul
EUR 240

[One Step] ARHGDIA Antibody Kit

RK05623 50 ul
EUR 240

[One Step] ATP5A1 Antibody Kit

RK05624 50 ul
EUR 240

[One Step] ATP5H Antibody Kit

RK05625 50 ul
EUR 240

[One Step] BCAP31 Antibody Kit

RK05626 50 ul
EUR 240

[One Step] BMP5 Antibody Kit

RK05627 50 ul
EUR 240

[One Step] C1QBP Antibody Kit

RK05628 50 ul
EUR 240

[One Step] CALB2 Antibody Kit

RK05629 50 ul
EUR 240

[One Step] CAT Antibody Kit

RK05630 50 ul
EUR 240

[One Step] CBX3 Antibody Kit

RK05631 50 ul
EUR 240

[One Step] CCT8 Antibody Kit

RK05632 50 ul
EUR 240

[One Step] CHCHD3 Antibody Kit

RK05633 50 ul
EUR 240

[One Step] CLDN7 Antibody Kit

RK05634 50 ul
EUR 240

[One Step] COPS5 Antibody Kit

RK05635 50 ul
EUR 240

[One Step] COX5A Antibody Kit

RK05636 50 ul
EUR 240

[One Step] COX5B Antibody Kit

RK05637 50 ul
EUR 240

[One Step] CSNK2A1 Antibody Kit

RK05638 50 ul
EUR 240

[One Step] CST3 Antibody Kit

RK05639 50 ul
EUR 240

[One Step] CTNNA1 Antibody Kit

RK05640 50 ul
EUR 240

[One Step] DARS Antibody Kit

RK05641 50 ul
EUR 240

[One Step] DISC1 Antibody Kit

RK05642 50 ul
EUR 240

[One Step] DNAJB1 Antibody Kit

RK05643 50 ul
EUR 240

[One Step] DNAJC19 Antibody Kit

RK05644 50 ul
EUR 240

[One Step] EIF5A Antibody Kit

RK05645 50 ul
EUR 240

[One Step] FDPS Antibody Kit

RK05646 50 ul
EUR 240

[One Step] FLOT1 Antibody Kit

RK05647 50 ul
EUR 240

[One Step] GDA Antibody Kit

RK05648 50 ul
EUR 240

[One Step] GOT2 Antibody Kit

RK05649 50 ul
EUR 240

[One Step] GPI Antibody Kit

RK05650 50 ul
EUR 240

[One Step] GSTP1 Antibody Kit

RK05651 50 ul
EUR 240

[One Step] HACE1 Antibody Kit

RK05652 50 ul
EUR 240

[One Step] HADHA Antibody Kit

RK05653 50 ul
EUR 240

[One Step] HAX1 Antibody Kit

RK05654 50 ul
EUR 240

[One Step] HK1 Antibody Kit

RK05655 50 ul
EUR 240

[One Step] HPCAL1 Antibody Kit

RK05656 50 ul
EUR 240

[One Step] HPRT1 Antibody Kit

RK05657 50 ul
EUR 240

[One Step] HSP90AA1 Antibody Kit

RK05658 50 ul
EUR 240

[One Step] HSP70 Antibody Kit

RK05659 50 ul
EUR 240

[One Step] Hsc70 Antibody Kit

RK05660 50 ul
EUR 240

[One Step] Hsp60 Antibody Kit

RK05661 50 ul
EUR 240

[One Step] KPNB1 Antibody Kit

RK05662 50 ul
EUR 240

[One Step] LSM4 Antibody Kit

RK05663 50 ul
EUR 240

[One Step] ERK2 Antibody Kit

RK05664 50 ul
EUR 240

[One Step] MCCC2 Antibody Kit

RK05665 50 ul
EUR 240

[One Step] MCM3 Antibody Kit

RK05666 50 ul
EUR 240

[One Step] MOGS Antibody Kit

RK05667 50 ul
EUR 240

[One Step] MRPL13 Antibody Kit

RK05668 50 ul
EUR 240

[One Step] NDUFA7 Antibody Kit

RK05669 50 ul
EUR 240

[One Step] NDUFB10 Antibody Kit

RK05670 50 ul
EUR 240

[One Step] NDUFS4 Antibody Kit

RK05671 50 ul
EUR 240

[One Step] NDUFV2 Antibody Kit

RK05672 50 ul
EUR 240

[One Step] NFU1 Antibody Kit

RK05673 50 ul
EUR 240

[One Step] OAT Antibody Kit

RK05674 50 ul
EUR 240

[One Step] P2RY14 Antibody Kit

RK05675 50 ul
EUR 240

[One Step] PARG Antibody Kit

RK05676 50 ul
EUR 240

[One Step] PDXK Antibody Kit

RK05677 50 ul
EUR 240

[One Step] PEBP1 Antibody Kit

RK05678 50 ul
EUR 240

[One Step] PHB Antibody Kit

RK05679 50 ul
EUR 240

[One Step] PIDD Antibody Kit

RK05680 50 ul
EUR 240

[One Step] POLR2H Antibody Kit

RK05681 50 ul
EUR 240

[One Step] PPIA Antibody Kit

RK05682 50 ul
EUR 240

[One Step] PPP3R1 Antibody Kit

RK05683 50 ul
EUR 240

[One Step] PQBP1 Antibody Kit

RK05684 50 ul
EUR 240

[One Step] PSMA2 Antibody Kit

RK05685 50 ul
EUR 240

[One Step] PSMA3 Antibody Kit

RK05686 50 ul
EUR 240

Examination of genetic and pharmacological tools to study the proteasomal deubiquitinating enzyme USP14 in the nervous system

Strategies for enhancing protein degradation have been proposed for treating neurological diseases associated with a decline in proteasome activity. A proteasomal deubiquitinating enzyme that controls substrate entry into proteasomes, ubiquitin-specific protease 14 (USP14), is an attractive candidate for therapies that modulate proteasome activity.
This report tests the validity of genetic and pharmacological tools to study USP14’s role in regulating protein abundance. Although previous studies implicated USP14 in the degradation of MAPT, TARDBP, and PRNP, the levels of these proteins were similar in our neurons cultured from wild type and USP14-deficient mice. Neither loss nor overexpression of USP14 affected the levels of these proteins in mice, implying that modifying the amount of USP14 is not sufficient to alter their steady-state levels.
However, neuronal overexpression of a catalytic mutant of USP14 showed that manipulating USP14’s ubiquitin-hydrolase activity altered the levels of specific proteins in vivo. Although pharmacological inhibitors of USP14’s ubiquitin-hydrolase activity reduced MAPT, TARDBP, and PRNP in culture, the effect was similar in wild type and USP14-deficient neurons, thus impacting their use for specifically evaluating USP14 in a therapeutic manner.
While examining how targeting USP14 may affect other proteins in vivo, this report showed that FASN, RELA, CTNNB1, and SNAP23 are reduced in USP14-deficient mice; however, loss of USP14 differentially altered the levels of these proteins in the liver and brain. As such, it is critical to more thoroughly examine how inhibiting USP14 alters protein abundance to determine if targeting USP14 will be a beneficial strategy for treating neurodegenerative diseases.

Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton.
Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library.
At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-10ug 10ug
EUR 156
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-1mg 1mg
EUR 2283
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-500ug 500ug
EUR 1613
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

C115-50ug 50ug
EUR 369
Description: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

CHIP Polyclonal Antibody

ES11301-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against CHIP from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

CHIP Polyclonal Antibody

ES11301-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against CHIP from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

CHIP Polyclonal Antibody

ABP58142-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of CHIP from Human, Mouse. This CHIP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHIP protein at amino acid sequence of 160-240

CHIP Polyclonal Antibody

ABP58142-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of CHIP from Human, Mouse. This CHIP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHIP protein at amino acid sequence of 160-240

CHIP Polyclonal Antibody

ABP58142-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of CHIP from Human, Mouse. This CHIP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHIP protein at amino acid sequence of 160-240

Anti-CHIP antibody

STJ192459 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to CHIP

Human CHIP Antibody

32585-05111 150 ug
EUR 261

CHIP, human recombinant

4898-25
EUR 349

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

P-2002 96 Reactions
EUR 904.05
Description: Ask the seller for details

Pospiviroid One-Step PCR kit

Oneq-A546-100R 100T
EUR 1610

Pospiviroid One-Step PCR kit

Oneq-A546-150R 150T
EUR 2205

Pospiviroid One-Step PCR kit

Oneq-A546-50R 50T
EUR 1175

Lactobacillus One-Step PCR kit

Oneq-A566-100D 100T
EUR 866

Lactobacillus One-Step PCR kit

Oneq-A566-150D 150T
EUR 981

Lactobacillus One-Step PCR kit

Oneq-A566-50D 50T
EUR 718

Lactococcus One-Step PCR kit

Oneq-A567-100D 100T
EUR 866

Lactococcus One-Step PCR kit

Oneq-A567-150D 150T
EUR 981

Lactococcus One-Step PCR kit

Oneq-A567-50D 50T
EUR 718

Leuconostoc One-Step PCR kit

Oneq-A569-100D 100T
EUR 866

Leuconostoc One-Step PCR kit

Oneq-A569-150D 150T
EUR 981

Leuconostoc One-Step PCR kit

Oneq-A569-50D 50T
EUR 718

Pediococcus One-Step PCR kit

Oneq-A571-100D 100T
EUR 866

Pediococcus One-Step PCR kit

Oneq-A571-150D 150T
EUR 981

Pediococcus One-Step PCR kit

Oneq-A571-50D 50T
EUR 718

Halobacillus One-Step PCR kit

Oneq-A635-100D 100T
EUR 866

Halobacillus One-Step PCR kit

Oneq-A635-150D 150T
EUR 981

Halobacillus One-Step PCR kit

Oneq-A635-50D 50T
EUR 718

Enterovirus One-Step PCR kit

Oneq-H433-100R 100T
EUR 1610

Enterovirus One-Step PCR kit

Oneq-H433-150R 150T
EUR 2205

Enterovirus One-Step PCR kit

Oneq-H433-50R 50T
EUR 1175

Mengovirus One-Step PCR kit

Oneq-H453-100R 100T
EUR 1610

Mengovirus One-Step PCR kit

Oneq-H453-150R 150T
EUR 2205

Mengovirus One-Step PCR kit

Oneq-H453-50R 50T
EUR 1175

Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
EUR 1610

Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
EUR 2205

Astrovirus One-Step PCR kit

Oneq-H491-50R 50T
EUR 1175

Calicivirus One-Step PCR kit

Oneq-H493-100R 100T
EUR 1610

Calicivirus One-Step PCR kit

Oneq-H493-150R 150T
EUR 2205

Calicivirus One-Step PCR kit

Oneq-H493-50R 50T
EUR 1175

Adenovirus One-Step PCR kit

Oneq-H501-100D 100T
EUR 866

Adenovirus One-Step PCR kit

Oneq-H501-150D 150T
EUR 981

Adenovirus One-Step PCR kit

Oneq-H501-50D 50T
EUR 718

Polyomavirus One-Step PCR kit

Oneq-H515-100D 100T
EUR 866

Polyomavirus One-Step PCR kit

Oneq-H515-150D 150T
EUR 981

Polyomavirus One-Step PCR kit

Oneq-H515-50D 50T
EUR 718

Ruminococcus One-Step PCR kit

Oneq-H619-100D 100T
EUR 866

Ruminococcus One-Step PCR kit

Oneq-H619-150D 150T
EUR 981

Ruminococcus One-Step PCR kit

Oneq-H619-50D 50T
EUR 718

Bifidobacterium One-Step PCR kit

Oneq-H621-100D 100T
EUR 866

Bifidobacterium One-Step PCR kit

Oneq-H621-150D 150T
EUR 981

Bifidobacterium One-Step PCR kit

Oneq-H621-50D 50T
EUR 718

Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
EUR 1610

Sapovirus One-Step PCR kit

Oneq-H630-150R 150T
EUR 2205

Sapovirus One-Step PCR kit

Oneq-H630-50R 50T
EUR 1175

Parechovirus One-Step PCR kit

Oneq-H642-100R 100T
EUR 1610

Parechovirus One-Step PCR kit

Oneq-H642-150R 150T
EUR 2205

Parechovirus One-Step PCR kit

Oneq-H642-50R 50T
EUR 1175

Metapneumovirus One-Step PCR kit

Oneq-H643-100R 100T
EUR 1610

Metapneumovirus One-Step PCR kit

Oneq-H643-150R 150T
EUR 2205

Metapneumovirus One-Step PCR kit

Oneq-H643-50R 50T
EUR 1175

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 866

Entamoeba One-Step PCR kit

Oneq-H683-150D 150T
EUR 981

Entamoeba One-Step PCR kit

Oneq-H683-50D 50T
EUR 718

Acanthamoeba One-Step PCR kit

Oneq-H684-100D 100T
EUR 866

Acanthamoeba One-Step PCR kit

Oneq-H684-150D 150T
EUR 981

Acanthamoeba One-Step PCR kit

Oneq-H684-50D 50T
EUR 718

Echovirus One-Step PCR kit

Oneq-H722-100D 100T
EUR 866

Echovirus One-Step PCR kit

Oneq-H722-150D 150T
EUR 981

Echovirus One-Step PCR kit

Oneq-H722-50D 50T
EUR 718

ranavirus One-Step PCR kit

Oneq-V337-100D 100T
EUR 866

ranavirus One-Step PCR kit

Oneq-V337-150D 150T
EUR 981

ranavirus One-Step PCR kit

Oneq-V337-50D 50T
EUR 718

Ruminococcus One-Step PCR kit

Oneq-V619-100D 100T
EUR 747.4
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-150D 150T
EUR 838.75
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-50D 50T
EUR 628.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit

Oneq-V621-100D 100T
EUR 747.4
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-150D 150T
EUR 838.75
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-50D 50T
EUR 628.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 267.5

[One Step] ACTR2 Antibody Kit

RK05617 50 ul
EUR 240

[One Step] ACTR3 Antibody Kit

RK05618 50 ul
EUR 240

[One Step] AK4 Antibody Kit

RK05619 50 ul
EUR 240

[One Step] AKR1A1 Antibody Kit

RK05620 50 ul
EUR 240