Exploring the Genetic Background of the Differences in Nest-Building Behavior in European Rabbit

  • Once a day, nursing and absentee mothering make the wild rabbit (Oryctolagus cuniculus) an ideal model animal for measuring differences in maternal behavior. Behavioral events and their hormonal regulation leading to parturition are well documented; however, the genetic background behind individual differences in this complex process is unknown.
  • Decreased progesterone hormone level and the reduction of progesterone receptor activity are crucial to initiating the collection of nest material. The progesterone receptor gene is a likely candidate affecting nest-building behavior. In addition to several known point mutations in the progesterone receptor gene of the European wild rabbit, we have found a new mutation in the promoter region of the gene at 2682 T > C.
  • Although this new single nucleotide polymorphism (SNP) was not involved in the formation of the nest-building behavior, an SNP (2464G > A) already described in the promoter region showed an association with individual differences in the initiation of hay carrying. The distribution of this SNP delivered an opposite result compared to domestic rabbits.
  • Genotype (GG) with high uterine capacity was most frequent; the hereditary value of the trait was h2 = 0.10. Thus, progesterone receptor gene polymorphism may manifest in individual differences affecting breeding success in this species.
informedchoiceaboutcancerscreening
informedchoiceaboutcancerscreening

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit 
P-2003
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  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

One Step RT PCR Kit
9K-005-0011 100Rxn, 100prep
EUR 321

Easy? One Step RT Kit
M1162-100 each
EUR 561.6

One Step RT-PCR Kit
4204A 125 Tests
EUR 200

One Step RT-PCR Kit
4204B 250 Tests
EUR 300

[One Step] HK1 Antibody Kit
MBS9145313-002mL 0.02mL
EUR 200

[One Step] HK1 Antibody Kit
MBS9145313-005mL 0.05mL
EUR 255

[One Step] HK1 Antibody Kit
MBS9145313-01mL 0.1mL
EUR 345

[One Step] HK1 Antibody Kit
MBS9145313-02mL 0.2mL
EUR 545

[One Step] HK1 Antibody Kit
MBS9145313-5x02mL 5x0.2mL
EUR 2265

[One Step] MIF antibody Kit
MBS9145322-002mL 0.02mL
EUR 235

[One Step] MIF antibody Kit
MBS9145322-005mL 0.05mL
EUR 295

[One Step] MIF antibody Kit
MBS9145322-01mL 0.1mL
EUR 425

[One Step] MIF antibody Kit
MBS9145322-02mL 0.2mL
EUR 600

[One Step] MIF antibody Kit
MBS9145322-5x02mL 5x0.2mL
EUR 2525

[One Step] RAN Antibody Kit
MBS9145352-002mL 0.02mL
EUR 200

[One Step] RAN Antibody Kit
MBS9145352-005mL 0.05mL
EUR 255

[One Step] RAN Antibody Kit
MBS9145352-01mL 0.1mL
EUR 345

[One Step] RAN Antibody Kit
MBS9145352-02mL 0.2mL
EUR 545

[One Step] RAN Antibody Kit
MBS9145352-5x02mL 5x0.2mL
EUR 2265

[One Step] AK4 Antibody Kit
MBS9145359-002mL 0.02mL
EUR 220

[One Step] AK4 Antibody Kit
MBS9145359-005mL 0.05mL
EUR 295

[One Step] AK4 Antibody Kit
MBS9145359-01mL 0.1mL
EUR 430

[One Step] AK4 Antibody Kit
MBS9145359-02mL 0.2mL
EUR 600

[One Step] AK4 Antibody Kit
MBS9145359-5x02mL 5x0.2mL
EUR 2525

[One Step] PHB Antibody Kit
MBS9145372-002mL 0.02mL
EUR 220

[One Step] PHB Antibody Kit
MBS9145372-005mL 0.05mL
EUR 295

[One Step] PHB Antibody Kit
MBS9145372-01mL 0.1mL
EUR 430

[One Step] PHB Antibody Kit
MBS9145372-02mL 0.2mL
EUR 600

[One Step] PHB Antibody Kit
MBS9145372-5x02mL 5x0.2mL
EUR 2525

[One Step] SRM antibody Kit
MBS9145380-002mL 0.02mL
EUR 220

[One Step] SRM antibody Kit
MBS9145380-005mL 0.05mL
EUR 295

[One Step] SRM antibody Kit
MBS9145380-01mL 0.1mL
EUR 430

[One Step] SRM antibody Kit
MBS9145380-02mL 0.2mL
EUR 600

[One Step] SRM antibody Kit
MBS9145380-5x02mL 5x0.2mL
EUR 2525

[One Step] OAT Antibody Kit
MBS9145397-002mL 0.02mL
EUR 235

[One Step] OAT Antibody Kit
MBS9145397-005mL 0.05mL
EUR 295

[One Step] OAT Antibody Kit
MBS9145397-01mL 0.1mL
EUR 420

[One Step] OAT Antibody Kit
MBS9145397-02mL 0.2mL
EUR 600

[One Step] OAT Antibody Kit
MBS9145397-5x02mL 5x0.2mL
EUR 2525

[One Step] GDA Antibody Kit
MBS9145406-002mL 0.02mL
EUR 200

[One Step] GDA Antibody Kit
MBS9145406-005mL 0.05mL
EUR 255

[One Step] GDA Antibody Kit
MBS9145406-01mL 0.1mL
EUR 345

[One Step] GDA Antibody Kit
MBS9145406-02mL 0.2mL
EUR 545

[One Step] GDA Antibody Kit
MBS9145406-5x02mL 5x0.2mL
EUR 2265

[One Step] GPI Antibody Kit
MBS9145412-002mL 0.02mL
EUR 200

[One Step] GPI Antibody Kit
MBS9145412-005mL 0.05mL
EUR 255

[One Step] GPI Antibody Kit
MBS9145412-01mL 0.1mL
EUR 345

[One Step] GPI Antibody Kit
MBS9145412-02mL 0.2mL
EUR 545

[One Step] GPI Antibody Kit
MBS9145412-5x02mL 5x0.2mL
EUR 2265

[One Step] VCP Antibody Kit
MBS9145423-002mL 0.02mL
EUR 200

[One Step] VCP Antibody Kit
MBS9145423-005mL 0.05mL
EUR 255

[One Step] VCP Antibody Kit
MBS9145423-01mL 0.1mL
EUR 345

[One Step] VCP Antibody Kit
MBS9145423-02mL 0.2mL
EUR 545

[One Step] VCP Antibody Kit
MBS9145423-5x02mL 5x0.2mL
EUR 2265

[One Step] AK4 Antibody Kit
RK05619 50 ul
EUR 88.56

[One Step] GDA Antibody Kit
RK05648 50 ul
EUR 88.56

[One Step] GPI Antibody Kit
RK05650 50 ul
EUR 88.56

[One Step] HK1 Antibody Kit
RK05655 50 ul
EUR 88.56

[One Step] OAT Antibody Kit
RK05674 50 ul
EUR 88.56

[One Step] PHB Antibody Kit
RK05679 50 ul
EUR 88.56

[One Step] RAN Antibody Kit
RK05695 50 ul
EUR 88.56

[One Step] VCP Antibody Kit
RK05721 50 ul
EUR 88.56

[One Step] MIF antibody Kit
RK05730 50 ul
EUR 88.56

[One Step] SRM antibody Kit
RK05732 50 ul
EUR 88.56

Multi One Step Cloning Kit
6006A 25 Tests
EUR 200

Multi One Step Cloning Kit
6006B 50 Tests
EUR 300

Multi One Step Cloning Kit
6006C 100 Tests
EUR 500

[One Step] PIDD Antibody Kit
MBS9145312-002mL 0.02mL
EUR 200

[One Step] PIDD Antibody Kit
MBS9145312-005mL 0.05mL
EUR 255

[One Step] PIDD Antibody Kit
MBS9145312-01mL 0.1mL
EUR 345

[One Step] PIDD Antibody Kit
MBS9145312-02mL 0.2mL
EUR 545

[One Step] PIDD Antibody Kit
MBS9145312-5x02mL 5x0.2mL
EUR 2265

[One Step] MCM3 Antibody Kit
MBS9145316-002mL 0.02mL
EUR 200

[One Step] MCM3 Antibody Kit
MBS9145316-005mL 0.05mL
EUR 255

[One Step] MCM3 Antibody Kit
MBS9145316-01mL 0.1mL
EUR 345

[One Step] MCM3 Antibody Kit
MBS9145316-02mL 0.2mL
EUR 545

[One Step] MCM3 Antibody Kit
MBS9145316-5x02mL 5x0.2mL
EUR 2265

[One Step] PYGB Antibody Kit
MBS9145317-002mL 0.02mL
EUR 200

[One Step] PYGB Antibody Kit
MBS9145317-005mL 0.05mL
EUR 255

[One Step] PYGB Antibody Kit
MBS9145317-01mL 0.1mL
EUR 345

[One Step] PYGB Antibody Kit
MBS9145317-02mL 0.2mL
EUR 545

[One Step] PYGB Antibody Kit
MBS9145317-5x02mL 5x0.2mL
EUR 2265

[One Step] TOP1 Antibody Kit
MBS9145318-002mL 0.02mL
EUR 200

[One Step] TOP1 Antibody Kit
MBS9145318-005mL 0.05mL
EUR 255

Examination of genetic and pharmacological tools to study the proteasomal deubiquitinating enzyme USP14 in the nervous system

Strategies for enhancing protein degradation have been proposed for treating neurological diseases associated with a decline in proteasome activity. A proteasomal deubiquitinating enzyme that controls substrate entry into proteasomes, ubiquitin-specific protease 14 (USP14), is an attractive candidate for therapies that modulate proteasome activity.
This report tests the validity of genetic and pharmacological tools to study USP14’s role in regulating protein abundance. Although previous studies implicated USP14 in the degradation of MAPT, TARDBP, and PRNP, the levels of these proteins were similar in our neurons cultured from wild type and USP14-deficient mice. Neither loss nor overexpression of USP14 affected the levels of these proteins in mice, implying that modifying the amount of USP14 is not sufficient to alter their steady-state levels.
However, neuronal overexpression of a catalytic mutant of USP14 showed that manipulating USP14’s ubiquitin-hydrolase activity altered the levels of specific proteins in vivo. Although pharmacological inhibitors of USP14’s ubiquitin-hydrolase activity reduced MAPT, TARDBP, and PRNP in culture, the effect was similar in wild type and USP14-deficient neurons, thus impacting their use for specifically evaluating USP14 in a therapeutic manner.
While examining how targeting USP14 may affect other proteins in vivo, this report showed that FASN, RELA, CTNNB1, and SNAP23 are reduced in USP14-deficient mice; however, loss of USP14 differentially altered the levels of these proteins in the liver and brain. As such, it is critical to more thoroughly examine how inhibiting USP14 alters protein abundance to determine if targeting USP14 will be a beneficial strategy for treating neurodegenerative diseases.

Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton.
Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library.
At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

Easy? One Step RT Kit
M1162-100 each
EUR 561.6

One Step RT-PCR Kit
4204A 125 Tests
EUR 200

One Step RT-PCR Kit
4204B 250 Tests
EUR 300

[One Step] HK1 Antibody Kit
MBS9145313-002mL 0.02mL
EUR 200

[One Step] HK1 Antibody Kit
MBS9145313-005mL 0.05mL
EUR 255

[One Step] HK1 Antibody Kit
MBS9145313-01mL 0.1mL
EUR 345

[One Step] HK1 Antibody Kit
MBS9145313-02mL 0.2mL
EUR 545

[One Step] HK1 Antibody Kit
MBS9145313-5x02mL 5x0.2mL
EUR 2265

[One Step] MIF antibody Kit
MBS9145322-002mL 0.02mL
EUR 235

[One Step] MIF antibody Kit
MBS9145322-005mL 0.05mL
EUR 295

[One Step] MIF antibody Kit
MBS9145322-01mL 0.1mL
EUR 425

[One Step] MIF antibody Kit
MBS9145322-02mL 0.2mL
EUR 600

[One Step] MIF antibody Kit
MBS9145322-5x02mL 5x0.2mL
EUR 2525

[One Step] RAN Antibody Kit
MBS9145352-002mL 0.02mL
EUR 200

[One Step] RAN Antibody Kit
MBS9145352-005mL 0.05mL
EUR 255

[One Step] RAN Antibody Kit
MBS9145352-01mL 0.1mL
EUR 345

[One Step] RAN Antibody Kit
MBS9145352-02mL 0.2mL
EUR 545

[One Step] RAN Antibody Kit
MBS9145352-5x02mL 5x0.2mL
EUR 2265

[One Step] AK4 Antibody Kit
MBS9145359-002mL 0.02mL
EUR 220

[One Step] AK4 Antibody Kit
MBS9145359-005mL 0.05mL
EUR 295

[One Step] AK4 Antibody Kit
MBS9145359-01mL 0.1mL
EUR 430

[One Step] AK4 Antibody Kit
MBS9145359-02mL 0.2mL
EUR 600

[One Step] AK4 Antibody Kit
MBS9145359-5x02mL 5x0.2mL
EUR 2525

[One Step] PHB Antibody Kit
MBS9145372-002mL 0.02mL
EUR 220

[One Step] PHB Antibody Kit
MBS9145372-005mL 0.05mL
EUR 295

[One Step] PHB Antibody Kit
MBS9145372-01mL 0.1mL
EUR 430

[One Step] PHB Antibody Kit
MBS9145372-02mL 0.2mL
EUR 600

[One Step] PHB Antibody Kit
MBS9145372-5x02mL 5x0.2mL
EUR 2525

[One Step] SRM antibody Kit
MBS9145380-002mL 0.02mL
EUR 220

[One Step] SRM antibody Kit
MBS9145380-005mL 0.05mL
EUR 295

[One Step] SRM antibody Kit
MBS9145380-01mL 0.1mL
EUR 430

[One Step] SRM antibody Kit
MBS9145380-02mL 0.2mL
EUR 600

[One Step] SRM antibody Kit
MBS9145380-5x02mL 5x0.2mL
EUR 2525

[One Step] OAT Antibody Kit
MBS9145397-002mL 0.02mL
EUR 235

[One Step] OAT Antibody Kit
MBS9145397-005mL 0.05mL
EUR 295

[One Step] OAT Antibody Kit
MBS9145397-01mL 0.1mL
EUR 420

[One Step] OAT Antibody Kit
MBS9145397-02mL 0.2mL
EUR 600

[One Step] OAT Antibody Kit
MBS9145397-5x02mL 5x0.2mL
EUR 2525

[One Step] GDA Antibody Kit
MBS9145406-002mL 0.02mL
EUR 200

[One Step] GDA Antibody Kit
MBS9145406-005mL 0.05mL
EUR 255

[One Step] GDA Antibody Kit
MBS9145406-01mL 0.1mL
EUR 345

[One Step] GDA Antibody Kit
MBS9145406-02mL 0.2mL
EUR 545

[One Step] GDA Antibody Kit
MBS9145406-5x02mL 5x0.2mL
EUR 2265

[One Step] GPI Antibody Kit
MBS9145412-002mL 0.02mL
EUR 200

[One Step] GPI Antibody Kit
MBS9145412-005mL 0.05mL
EUR 255

[One Step] GPI Antibody Kit
MBS9145412-01mL 0.1mL
EUR 345

[One Step] GPI Antibody Kit
MBS9145412-02mL 0.2mL
EUR 545

[One Step] GPI Antibody Kit
MBS9145412-5x02mL 5x0.2mL
EUR 2265

[One Step] VCP Antibody Kit
MBS9145423-002mL 0.02mL
EUR 200

[One Step] VCP Antibody Kit
MBS9145423-005mL 0.05mL
EUR 255

[One Step] VCP Antibody Kit
MBS9145423-01mL 0.1mL
EUR 345

[One Step] VCP Antibody Kit
MBS9145423-02mL 0.2mL
EUR 545

[One Step] VCP Antibody Kit
MBS9145423-5x02mL 5x0.2mL
EUR 2265

[One Step] AK4 Antibody Kit
RK05619 50 ul
EUR 88.56

[One Step] GDA Antibody Kit
RK05648 50 ul
EUR 88.56

[One Step] GPI Antibody Kit
RK05650 50 ul
EUR 88.56

[One Step] HK1 Antibody Kit
RK05655 50 ul
EUR 88.56

[One Step] OAT Antibody Kit
RK05674 50 ul
EUR 88.56

[One Step] PHB Antibody Kit
RK05679 50 ul
EUR 88.56

[One Step] RAN Antibody Kit
RK05695 50 ul
EUR 88.56

[One Step] VCP Antibody Kit
RK05721 50 ul
EUR 88.56

[One Step] MIF antibody Kit
RK05730 50 ul
EUR 88.56

[One Step] SRM antibody Kit
RK05732 50 ul
EUR 88.56

Multi One Step Cloning Kit
6006A 25 Tests
EUR 200

Multi One Step Cloning Kit
6006B 50 Tests
EUR 300

Multi One Step Cloning Kit
6006C 100 Tests
EUR 500

[One Step] PIDD Antibody Kit
MBS9145312-002mL 0.02mL
EUR 200

[One Step] PIDD Antibody Kit
MBS9145312-005mL 0.05mL
EUR 255

[One Step] PIDD Antibody Kit
MBS9145312-01mL 0.1mL
EUR 345

[One Step] PIDD Antibody Kit
MBS9145312-02mL 0.2mL
EUR 545

[One Step] PIDD Antibody Kit
MBS9145312-5x02mL 5x0.2mL
EUR 2265

[One Step] MCM3 Antibody Kit
MBS9145316-002mL 0.02mL
EUR 200

[One Step] MCM3 Antibody Kit
MBS9145316-005mL 0.05mL
EUR 255

[One Step] MCM3 Antibody Kit
MBS9145316-01mL 0.1mL
EUR 345

[One Step] MCM3 Antibody Kit
MBS9145316-02mL 0.2mL
EUR 545

[One Step] MCM3 Antibody Kit
MBS9145316-5x02mL 5x0.2mL
EUR 2265

[One Step] PYGB Antibody Kit
MBS9145317-002mL 0.02mL
EUR 200

[One Step] PYGB Antibody Kit
MBS9145317-005mL 0.05mL
EUR 255

[One Step] PYGB Antibody Kit
MBS9145317-01mL 0.1mL
EUR 345

[One Step] PYGB Antibody Kit
MBS9145317-02mL 0.2mL
EUR 545

[One Step] PYGB Antibody Kit
MBS9145317-5x02mL 5x0.2mL
EUR 2265

[One Step] TOP1 Antibody Kit
MBS9145318-002mL 0.02mL
EUR 200

[One Step] TOP1 Antibody Kit
MBS9145318-005mL 0.05mL
EUR 255

[One Step] TOP1 Antibody Kit
MBS9145318-01mL 0.1mL
EUR 345

[One Step] TOP1 Antibody Kit
MBS9145318-02mL 0.2mL
EUR 545

[One Step] TOP1 Antibody Kit
MBS9145318-5x02mL 5x0.2mL
EUR 2265

[One Step] PARG Antibody Kit
MBS9145319-002mL 0.02mL
EUR 200